In another embodiment, the present invention is directed toward a modified TetR(A) protein comprising an amino acid substitution at a position selected from the group consisting of positions 59, 70, 71, 91, 95, 96, 99, 100, 101, 103, 114, 127, 158, 159, 160, 179, 193, 197, and 201 of the TetR(A) protein as depicted in SEQ ID NO: 34, wherein said modified TetR(A) protein binds a TetR(A) operator sequence with greater affinity in the presence of tetracycline than in the absence of tetracycline; wherein: the amino acid substitution at position 59 is asparagine; the amino acid substitution at position 70 is valine; the amino acid substitution at position 71 is valine; the amino acid substitution at position 91 is glutamine; the amino acid substitution at position 95 is selected from the group consisting of glycine and glutamic acid; the amino acid substitution at position 95 is glycine; the amino acid substitution at position 95 is glutamic acid; the amino acid substitution at position 96 is arginine; the amino acid substitution at position 96 is glutamic acid; the amino acid substitution at position 99 is glutamic acid; the amino acid substitution at position 100 is alanine; the amino acid substitution at position 101 is histidine; the amino acid substitution at position 103 is serine; the amino acid substitution at position 114 is valine; the amino acid substitution at position 127 is selected from the group consisting of arginine, lysine, and histidine; the amino acid substitution at position 158 is cysteine; the amino acid substitution at position 159 is leucine; the amino acid substitution at position 160 is valine; the amino acid substitution at position 179 is valine; the amino acid substitution at position 193 is glycine; the amino acid substitution at position 197 is tryptophan; and the amino acid substitution at position 201 is histidine.
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WHY BECOME A MEMBER?
There are a wide variety of reasons for joining a Chamber such as ours. Even in this age of technology, as a new business, do not underestimate the power of building relationships face to face through attending networking events. It is not easy to walk into an event but you will soon feel at home and we will do all we can to help you make the right contacts. Whatever the size of your business you will have access to a range of benefits all geared to advising, helping and developing. We support local business relationships and in turn our local community.
At the heart of being a Chamber member is the opportunity to network with other businesses. Through networking we not only receive business leads but we form strong relationships within the local community allowing us opportunities to be consulted on and become involved. We are currently hosting a series of Happy Hours usually at The Dolphin Pub for all our members and potential members to come along and meet each other. These take place on the 1st Thursday of the month.
Real networking should be organic and grow, the more contacts you make the wider the opportunities for business spread. Trust and familiarity are still as important in commerce as they ever were in the ‘old school network’ of bygone years. The only difference now is we belong to a shared interest group rather than rely on our history or family.
Our town your business
We love to support The Town and its many groups and organisations; The Town Team, The Town Meadow Group and the Town Council, Three Rivers Partnership etc. We love to support local events such as Gillingham in Gear, Next Steps Apprenticeship Evening at the School, and Gillingham Town Council’s Lighting Up Festival. We love to hear about and support new ventures so please get involved.
Support, contribute and develop
We have a very industrious town with some amazing and interesting industries to shout about. Some traditional bricks and mortar companies work alongside new internet and virtual businesses. The town has grown from its small origins to a thriving business community in all its guises and many of those companies are members of Chamber. Many of them support us with our mission and values for our town and we in turn promote them as well as raising their profile across online platforms such as this website and a full social media programme.
Whatever the next two weeks bring Gillingham Chamber of Commerce and Industry ar...
19 October, 2019
A note from the Chairman of the Gillingham Chamber of Commerce
Gillingham Chamber of commerce is on the up, with plenty of new members continuing to join us. During the past six months we have welcomed new business to our Town and unfortunately seen the closure of a couple of high street shops; We are sad to see the closure of Gilyard Scarth, The Cats Protection league, Charlies and the departure of Arnie and Sarah from the Wine bar, our regular Networking Meeting place.
We extend a warm welcome to following New Businesses:
The New Donkey Sanctuary shop they have open in Gilyard Scarth’s vacated premises all proceeds taken in this shop go towards looking after saved animals at their local farm Locks Lane, Gillingham.
Anne Hitchcock is looking to reopen the downstairs of the Slade Building in the square and is seeking ideas from organisations and the public through open days to invite people in to view the building and suggest what type of business the towns residents feel should be there; Anne explained she is restoring the building to its former glory.
Further into The Square there is a new Beauticians opening and a Child-care centre in the former Topsie Rabbit Building at Coachman’s Corner.
Folks it is very important to continue to support and use the local businesses to keep the local economy buoyant.
Our Network Happy Hour Evenings are always held on the first Thursday of the month. We welcome fellow business owners to join us in our New Meeting place The Dolphin Pub from 6pm for a Happy Hour Event to have a chat and a chance to network so please drop in some months we have guest speakers who give a short 20 minute informative talk which have been very popular; Different companies sponsor the 1st
drink and are offered a short 5 minutes if they wish to talk about the business.
Gillingham carnival will be on 5th
October, the Carnival Committee request that various shops participate in the Window Spotting Competition. Forms will be available from Cullingford Carpets and forms can be dropped back to Cullingfords; this event is always very well attended, seeing large numbers of people standing in our High Street, it’s a great opportunity for those businesses on the High Street to get the Windows dressed full of the Goods that you offer giving the people of Gillingham something to look at while they wait for the Carnival Parade to start.
The Chamber are organising a Race Night on the 22nd
November, Tickets are £10.00 each full details can be obtained from our membership secretary email@example.com
who will be happy to discuss the event with you.
On the 29th
November will see The Festive Light Switch on, this is an event that will have a parade through the High Street, to see the large Christmas Tree being sponsored by Our members Station Road Garage switched on, It will be lovely to request all our High Street Businesses with their Windows and Door ways dressed and lights switched on to illuminate the Highstreet on the eve 29th; Why not offer a late night opening and invite Customers in for a Mince Pie and complimentary drink and the opportunity to purchase goods from your shops. This Event is extremely popular and always attended by hundreds of people, too good an opportunity to miss passing trade, by staying closed.
The Council will be providing lights throughout the town and the Christmas Trees mounted above shops to give a fantastic Christmas Atmosphere during the festive period.
On the 5th
December the Chamber will be organising a Christmas Dinner in place of the Network Evening at the Dolphin Pub, Tickets will be limited, we shall be sending out details shortly.
Lastly, we were sad to see the resignation of Janet Pegram from our committee, we wish Janet well and thank Janet for the time she was on our committee and wish her well for the future.